黑帮老大艾迪?金策划了一起凶杀案，被偶然路过的年轻人西恩?琼斯（Nathan Phillips 饰）目击
为了在法庭上成功指控这个作恶多端的黑社会头子，警方派出资深联邦密探尼维利?费恩（Samuel L Jackson 饰）负责保护西恩，并将其从夏威夷护送至洛杉矶法庭 为避免证人遇害，尼维利采取声东击西的策略，与西恩偷偷乘上一架普通的红眼航班
然而这项绝密的计划被艾迪安排在警方的内奸获知，为了逃脱法律制裁，艾迪派出携带大量毒蛇的杀手登上飞机，一场离奇恐怖的血腥灭口案件就此展开……
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